Examination of honey from five different stingless bee species across Neotropical and Indo-Australian regions has enabled for the first time the identification of the unusual disaccharide trehalulose as a major component representing between 13 and 44 g per 100 g of each of these honeys. The previously unrecognized abundance of trehalulose in stingless bee honeys is concrete evidence that supports some of the reported health attributes of this product.